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KMID : 0390220020130010085
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Journal of Clinical Otolaryngology, Head and Neck Surgery
2002 Volume.13 No. 1 p.85 ~ p.92
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Expression of Nitric Oxide Synthases in Nasal Polyp
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Park Seong-Kook
Kim Dong-Kyoon
Heo Kyung-Wook
Yang Young-Il
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Abstract
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Background and Objective : The patogenesis of nasal polyp is poorly understood. Nitric oxide (NO) plays a major role in a number of physiologic function and may be cytotoxic in high concentrations. No is formed by the oxidative deamination of L-arginine by nitric oxide synthases (NOS). Three NOS isoforms have been identified in human. Constitutive forms of NOS are present in vascular endothelial cells (Type Ⅲ NOS, eNOS) and neurons in the brain and the peripheral nervous system (Type ⅠNOS, nNOS) shereas the inducible form smooth-muscls cells, and fibroblasts. The aim of this study was to detect and localize three Nos isoforms expression in nasal polyp tissues, and compare these findins with inferior nasal turbinate tissues. Materials and Methods : The authors examined the expression and localization of three NOS isoforms in nasal mucosal specimens from patients undergone elective nasal tubinectomy (n=10) and nasal polypectomy (n=23). The mRNA expressions of three NOS isoforms were determined by semi-quantitative reverse transcription-poly-merase chain reaction (RT-PCR) followed by Southern hybridization. The protein expression of theree NOS isofroms were examined by immunohistochemistry. Statistics were analysed using Wilcoxon rank sum test. Results : Semi-Quantitative RT-PCR southern analysis of RNA obtained from 23 surgical specimens of nasal polyps demonstrated the the mRNA expressions of iNOS and eNOS were significantly increased in nasal polyps turbinates. The immunogistochemical studies revealed that the immunoreactivity to iNOS protein was mainly localized to epithelium, whereas eNOS protein to vascular endothelium, and nNOS protein to inflammatory cell, eqithelium and vascular endothelium in all sqcimens reviewed. The high level of expression of three NOS isoforms in the nasal polyps were demonstrated in this study. Conclusion : The authors suggest that iNOS mRNA, eNOS mRNA and their product, nitric oxide may play an important role in the formation and growth of nasal polyps.
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KEYWORD
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Nitric oxide synthase, Nasal polyp, RT-PCR/southern blot, Immunohistochemistry
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